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. 2022 Jul 11;63(8):5. doi: 10.1167/iovs.63.8.5

Figure 7.

Figure 7.

TOMM20-containing cytoplasmic vesicles express LIMP-2 prior to full AQP5 plasma membrane insertion in bovine lens cortical fiber cells. (A-1–C-1) High-magnification confocal images of TOMM20 immunolabeling (red) and LIMP-2 immunolabeling (green) in the peripheral outer cortex (A), medial outer cortex (B), and outer cortex–inner cortex transitional region (C) of the bovine lens (Fig. 3A). In the peripheral outer cortex, LIMP-2 is faintly expressed (A) in cytoplasmic vesicles (A–C, striped arrowheads). TOMM20-containing cytoplasmic vesicles (closed arrowheads) in the peripheral outer cortex partially colocalize with LIMP-2. LIMP-2 expression is upregulated with fiber cell differentiation and simultaneously increased in TOMM20-containing cytoplasmic vesicles. LIMP-2 colocalizes with the TOMM20-containing cytoplasmic vesicles in the medial outer cortex and the outer cortex–inner cortex transitional region (B and C). (A-2–C-2) Enlarged images of the TOMM20-containing cytoplasmic vesicles indicated by the arrowheads in A-1, B-1, and C-1. (A-3–C-3) Replicate images of A-2, B-2, and C-2 with only LIMP-2 immunolabeling and DAPI labeling displayed. Scale bars: 10 µm (A-1, B-1, C-1) and 5 µm (A-2, A-3, B-2, B-3, C-2, C-3).