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. 2022 Jul 11;63(8):5. doi: 10.1167/iovs.63.8.5

Figure 8.

Figure 8.

AQP5-containing cytoplasmic vesicles are congruent in subcellular localization with vesicular assemblies identified via TEM analysis in bovine lens cortical fiber cells. (A) High-resolution, confocal images of AQP5 immunofluorescence (green) in the medial outer cortex of the bovine lens. Plasma membranes (PM) are labeled with WGA (red) (A-1) or connexin 50 (red) (A-2). Cellular nuclei (N) are labeled with DAPI staining (blue). AQP5-containing cytoplasmic vesicles (arrowheads) are distinctive among fiber cell cytoplasmic vesicles in the bovine lens outer cortex (Fig. 4). AQP5-containing cytoplasmic vesicles are frequently in close proximity or apposed to fiber cell plasma membranes (A-1) and cellular nuclei (A-2). The structures indicated by open arrowheads in A-1 and A-2 are enlarged and shown in A-3 and A-4, respectively. Scale bars: 5 µm (A-1, A-2) and 1 µm (A-3, A-4). (B) TEM images depicting vesicular assemblies of interest (arrows) in outer cortical fiber cells of the bovine lens in close proximity or apposed to the plasma membrane (B-1, B-3) or the nucleus (B-2, B-4). The structures indicated with arrows in B-1 and B-2 are enlarged and shown in B-3 and B-4, respectively. Scale bars: 500 nm (B-1, B-2) and 250 nm (B-3, B-4).