Figure 6.

(a) Irradiation of allyl-piperacillin 16 resulting in the formation of the antimicrobial and a fluorescent reporter of payload release. (b) Fluorescence response of allyl-piperacillin 16 upon irradiation (20 μM, water/acetonitrile 8:2, irradiation at λ_max = 400 nm, fluorescence excitation at λ = 434 nm, readout at λ = 565 nm). (c) Results of the antimicrobial assay. The predicted piperacillin concentrations calculated from the fluorescence response are reported, as well as the irradiation time (in seconds). Blue bars show bacterial growth, indicating piperacillin concentrations below the MIC. Red bars show full growth inhibition, indicating piperacillin concentrations above the MIC.