Figure 6.

Varying time intervals between two inputs. Darker and lighter bars correspond to experiment and simulation of output concentration at 60 min after the second input was added, respectively. Δt = |t_A – t_B|. All gates, reporters, and inputs were at 100, 150, and 90 nM, respectively. Data were averaged over three independent experiments using varying qualities of the gate molecules (unpurified vs gel purified). Error bars indicate standard deviation of the mean. Dashed line marks the separation between ON and OFF states. An example set of kinetics data is shown in Figure S5.