Newly generated cells display neuronal electrophysiological properties. a, Fluorescent micrographs of a GFP+ cell filled with Alexa through a recording pipette. b, Membrane potential in response to depolarizing currents (Im; 500 ms, 20–400 pA) recorded under current clamp at the resting potential (−73.5 mV). Numbers on the left indicate stimulus size. Scale: 25 mV, 50 ms. c, Action potential recorded at 80 pA. d, Firing rate versus current curve. e, Spontaneous postsynaptic currents recorded under voltage clamp (−80 mV). Scale: 20 pA, 100 ms. f, Confocal micrographs taken from a single plane (1 µm) after fixation of the slice. g, Overview of the dorsal blade of the granule cell layer (merged z-series of 23 planes).