Figure 1.

Spike-mRNA-immunized mice elicit antibodies against diverse regions of spike, several of which bound diverse beta-coronavirus spikes and one of which, WS6, neutralized them

(A) Immunization scheme. NatSP is the full transmembrane-containing native sequence of spike WA-1 strain; S-dTM is the soluble spike protein residues 1–1,206 of wild-type WA-1 strain.

(B) Binding of isolated antibodies by ELISA to subdomains of the SARS-CoV-2 spike. Purified monoclonal antibodies from hybridoma supernatants were analyzed for binding to SARS-CoV-2 S-dTM, S1 (S1-short and S1R), RBD, and NTD by ELISA.

(C) Binding of isolated antibodies assessed by ELISA to diverse beta-coronavirus pre-fusion-stabilized spikes (S2Ps).

(D) Neutralization assessment of hybridoma antibodies against SARS-CoV-2 WA-1 and SARS-CoV pseudoviruses on 293T-ACE2 cells.

See also Figure S1.