Fig. 7.

LncRNA TUG1 suppressed H/R-induced ferroptosis in HK-2 cells by regulating SRSF1/ACSL4. A Cell viability was assessed by CCK-8 kits. B Cell death of HK-2 cells was measured by TUNEL assay. TUNEL-positive cell quantitative bar diagrams are shown. Scale bar: 50 µm. C The levels of MDA and GSH in the supernatants of HK-2 cells were detected by commercial kits. D The levels of Fe²⁺ in HK-2 cells were checked by commercial kits. E The levels of ROS in HK-2 cells were detected using DCFH-DA probe. F The protein levels of ACSL4, GPX4, FTH1, and COX2 in HK-2 cells were assessed by western blot and shown as blot image and quantitative bar diagram. N = 3 samples per groups. *p < 0.05, **p < 0.01, ***p < 0.001. The above data were all measurement data and expressed as means ± SD. All experiment was performed three times