Fig. 8.

LncRNA TUG1 ameliorates IRI-induced kidney injury by suppressing ACSL4-mediated ferroptosis. A The expression of TUG1 and ACSL4 was measured in kidney tissues via qPCR. B Serum levels of creatinine and BUN were assessed by colorimetric method. C The injury and cell death of kidney tissues shown by HE dyes and TUNEL assay. Representative images of kidney H&E dyes and TUNEL staining was presented, and the kidney injury score and TUNEL-positive cell quantitative bar diagrams are shown. HE, scale bar: 200 µm; TUNEL, scale bar: 100 µm. D The levels of MDA and GSH in the mice were assessed by using commercial kits. E The levels of Fe²⁺ in the mice were checked by using commercial kits. F The protein levels of ACSL4, GPX4, FTH1, and COX2 in the kidney tissues were assessed by western blot and shown as blot image and quantitative bar diagram. N = 6 mice per groups. *p < 0.05, **p < 0.01, ***p < 0.001. The above data were all measurement data and expressed as means ± SD