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. 2022 Jul 15;21:146. doi: 10.1186/s12943-022-01607-8

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — CircPOLR2A promoted malignant biological properties in cRCC cells. a, b The expression of circPOLR2A and POLR2A mRNA was detected after circPOLR2A knockdown (a) or overexpression (b) in cRCC cells. c, d CCK-8 assay indicated the growth rate of cRCC cells transfected with lentivirus for circPOLR2A knockdown (c) or overexpression (d). Two-way ANOVA was used to evaluate significant differences. e Colony formation assay for cRCC cells. f EdU incorporation assay was performed to assess DNA synthesis in cRCC cells. Red fluorescence represented EdU-positive cells, while blue fluorescence represented total cells. Scale bar, 100 μm. g Cell migration and invasion assays of cRCC cells treated with circPOLR2A knockdown or overexpression. Left, transwell assay, scale bar, 40 μm; right, wound healing assay, scale bar, 100 μm. h Statistical analysis of the cell migration and invasion assays. i, j The effect of circPOLR2A knockdown or overexpression on cell apoptosis via flow cytometry assay