Fig. 5.

CircPOLR2A accelerated ubiquitin/proteasome-dependent protein degradation of PEBP1. a, b Protein level of PEBP1 in cRCC cells treated with 20 μM MG132 for 12 h. 5a, 786O cells transfected with circPOLR2A shRNA and control; 5b, Caki1 cells transfected with lentivirus for circPOLR2A overexpression and control. c, d Ubiquitination modification of PEBP1 proteins in cRCC cells was determined via Co-IP and western blotting. e, f EdU incorporation and colony formation assays were carried out for rescue experiments. 5e, the restoring effect of siPEBP1 on circPOLR2A downregulation in 786O cells; 5f, the restoring effect of ectopic PEBP1 expression on circPOLR2A upregulation in Caki1 cells. Scale bar, 100 μm