Fig. 2.

Knockdown of PDIA3P1 exacerbates DNA damage and proliferation inhibition induced by TMZ intervention. A B Representative images and quantification of comet assay showing the DNA damage caused by PDIA3P1 knockdown or vehicle control with or without TMZ treatment (400 μM, 48 h) on U118MG (A) and U87MG (B) cells. Scale bars, 100 μm. C D Representative images and quantification of γ-H2AX staining on U118MG (C) and U87MG (D) cells with or without TMZ treatment (400 μM, 48 h). Scale bars, 10 μm. E Representative images of U118MG cells subjected to the EdU cell proliferation assay (upper panel; scale bar, 100 μm) and quantification of EdU-positive cells (lower panel) with or without TMZ treatment (400 μM, 48 h). F Apoptosis assay showing the effect of PDIA3P1 knockdown on U118MG with or without TMZ treatment (400 μM, 48 h). The lower panel was the quantification of apoptosis cells