Table 1.
Oligonucleotide primers in real-time quantitative PCR.
| Gene | Forward primer | Reverse primer |
|---|---|---|
| iNOS | TCCTCAGGCTTGGGTCTTGT | ATCCTGTGTTGTTGGGCTGG |
| IL-6 | AGGATACCACCCACAACAGACC | TTGCCATTGCACAACTCTTTTC |
| TNF-α | AACAAGGAGGAGAAGTTCCCAAA | CTCCTCCGCTTGGTGGTTT |
| IL-1β | GCTTCCTTGTGCAAGTGTCT | TCTGGACAGCCCAAGTCAAG |
| Arg1 | CAAGCCAAAGCCCATAGAGATT | CATTGGCTTTTCCCACAGACC |
| IL-10 | CGACGCTGTCATCGATTTCTC | CAGTAGATGCCGGGTGGTTC |
| CD163 | GACAGACCCAACGGCTTACA | GGTCACAAAACTTCAACCGGA |
| TGF-β | CTGCTGACCCCCACTGATAC | AGCCCTGTATTCCGTCTCCT |
| GAPDH | AGTGCCAGCCTCGTCTCATA | GGTAACCAGGCGTCCGATAC |
| miR-23a-3p | AGATCACATTGCCAGGGAT | miScript Universal Primera |
| miR-130a-3p | CAGCAGTGCAATGTTAAAAGG | miScript Universal Primera |
iNOS, inducible nitric oxide synthase; IL-6, Interleukin 6; TNF-α, tumor necrosis factor α; IL-1β, Interleukin 1β; Arg1, arginase 1; IL-10, Interleukin 10; TGF-β, transforming growth factor β; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
aThe miScript Universal Primer in the miScript SYBR Green PCR Kit was used as the reverse primer.