Table 2.
Details of antibodies used for western blot
| Antibody target | Dilution | Species specificity | Source | Supplier | Catalog no. |
|---|---|---|---|---|---|
| MCL1 | 1:1000 | Human | Rabbit | Cell signaling Technology (Danvers MA, USA) | CS-5453 |
| BCL-XL | 1:1000 | Human | Rabbit | Cell signaling Technology | CS-2762 |
| PARP | 1:1000 | Human | Rabbit | Cell signaling Technology | CS-9532 |
| Full-length Caspase 3 | 1:1000 | Human | Rabbit | Cell signaling Technology | CS-14220 |
| Cleaved Caspase 3 | 1:1000 | Human | Rabbit | Cell signaling Technology | CS-9664 |
| RIPK1 | 1:1000 | Human | Rabbit | Cell signaling Technology | CS-14993 |
| MLKL | 1:1000 | Human | Rabbit | Cell signaling Technology | CS-3493 |
| Total p44/42 MAPK | 1:5000 | Human | Rabbit | Millipore (Burlington, MA, USA) | ABS44 |
| HRP-linked IgG | 1:10,000 | Anti-rabbit | Donkey | Jackson immunoResearch (West Grove PA, USA) | 711–035-152 |