Fig. 3.

MSC-sEV protected RPE cells against HG conditions in vitro. A The internalization of PKH-26-labeled MSC-sEV by RPE cells was detected by a confocal microscope. Scale bars, 25 μm. B Immunofluorescence analysis of Ki-67 in RPE cells. Scale bars, 25 μm. C CCK8 assay for the proliferation ability of RPE cells (n = 4). D Western blot analysis for the expressions of PCNA, Bcl-2 and Bax in RPE cells. E Detection of ROS generation in RPE cells. Scale bars, 100 μm. F MDA and SOD level measurement in RPE cells (n = 3). G Western blot analysis for the expressions of GPX1, NQO1, HO-1 and NRF2 in RPE cells. H Immunofluorescence staining of NRF2 in RPE cells. Scale bars, 25 μm. All data are presented as means ± SEM. ns, not significant, ^*P < 0.05, ^**P < 0.01 and ^***P < 0.001