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. 2022 Jul 15;13:311. doi: 10.1186/s13287-022-02979-w

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Cultivation and characterization of hDPSCs. A Primary hDPSCs at day 3. Scale bar = 100 μm. B hDPSCs at passage 3. Scale bar = 100 μm. C Flow cytometry analysis showing negative expression of CD34 and CD45 and positive expression of CD29, CD73 and CD90 in hDPSCs. D-F Multiple differentiations of hDPSCs. Alizarin red staining assayed osteo/odontogenic differentiation. Scale bar = 100 μm. Oil red O staining assayed adipogenic differentiation. Scale bar = 50 μm. Alcian blue staining assayed chondrogenic differentiation. Scale bar = 100 μm