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. Author manuscript; available in PMC: 2022 Jul 15.
Published in final edited form as: Methods Mol Biol. 2021;2276:357–382. doi: 10.1007/978-1-0716-1266-8_27

Table 7.

LC/MS sequence setup for sample injection order

Injection number Sample MS acquisition method Acquisition scheme
1 Solvent Blank 1 FS System Conditioning
2 Solvent Blank 2 FS System Conditioning
3 Reference Metabolite Mix 1 FS Qualitative Assessment
4 Solvent Blank 3 FS System Conditioning
5 Solvent Blank 4 FS System Conditioning
6 Pooled Sample Conditioning 1 FS System Conditioning
7 Pooled Sample Conditioning 2 FS System Conditioning
8 Pooled Sample Conditioning 3 FS System Conditioning
9 Pooled Sample 1 FS MS1 Profiling
10 Experimental Sample 1 FS MS1 Profiling
11 Experimental Sample 2 FS MS1 Profiling
12 Experimental Sample 3 FS MS1 Profiling
13 Experimental Sample 4 FS MS1 Profiling
14 Experimental Sample 5 FS MS1 Profiling
15 Experimental Sample 6 FS MS1 Profiling
16 Experimental Sample 7 FS MS1 Profiling
17 Experimental Sample 8 FS MS1 Profiling
18 Experimental Sample 9 FS MS1 Profiling
19 Experimental Sample 10 FS MS1 Profiling
20 Pooled Sample 2 FS MS1 Profiling
21 Experimental Sample 11 FS MS1 Profiling
… … … …
63 Experimental Sample 50 FS MS1 Profiling
64 Pooled Sample 6 FS MS1 Profiling
65 Solvent Blank 5 FS System Conditioning
66 Solvent Blank ID FS Background Ion ID
67 Pooled Plasma ID 00 FS System Conditioning
68 Pooled Sample ID 01 DDA Unknown Annotation
69 Reference Metabolite Mix 2 FS Qualitative Assessment
70 Solvent Blank 6 FS System Conditioning