Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Nov 16;12(4):e86. doi: 10.1002/cpch.86

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 The Authors.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Spectra assignment and stoichiometric measurement of the modified peptide. (A) Search strategy to determine the probe‐labeled site from isoTOP ABPP data. To determine the peptide containing the modification, MS1 searches for the coeluting pair with the specified mass difference are performed. To determine the residue labeled, MS2 spectra are assigned by de novo sequencing. (B) Scheme of stoichiometry measurement for modified peptide. Using a known quantity of synthetic standards of modified and total protein allows the calculation of fractional occupancy.