Skip to main content
. 2021 Sep 1;33(10):e13026. doi: 10.1111/jne.13026

FIGURE 1.

FIGURE 1

Effects of i.c.v. administration of FGF21 on expression of c‐Fos protein in tyrosine hydroxylase‐positive cells in the nucleus tractus solitarius and area postrema. Photographs of sections containing cells expressing c‐Fos protein‐positive nuclear profiles (black nuclear profiles) and tyrosine hydroxylase‐immunoreactive (TH‐ir) cytoplasmic profiles (brown profiles) in the nucleus tractus solitarius or area postrema of 1000 ng FGF21‐injected and vehicle‐injected rats (left columns). The number of tyrosine hydroxylase‐positive cells expressing c‐Fos protein in the nucleus tractus solitarius and the number of tyrosine hydroxylase‐positive cells expressing c‐Fos protein in the area postrema are shown (right column). Intracerebroventricular administration of FGF21 at a dose of 1000 ng increased the number of tyrosine hydroxylase‐positive cells expressing c‐Fos protein, suggesting that FGF21 activated catecholaminergic cells in the medulla oblongata. Error bars indicate the SEM. The number of animals injected with 0, 10, 100 and 1000 ng FGF21 was 6, 6, 6 and 5, respectively. Scale bars = 10 µm. *P < 0.05, **P < 0.01 vs control animals. Kruskal‐Wallis test followed by post‐hoc Dunn's test