FIGURE 11.

Anxiety‐related behaviours in an elevated plus maze test and social avoidance behaviours and immobility behaviours in a forced swimming test in mice injected with AAV‐hrGFP or AAV‐FGF21. A, Total distance of locomotion (Total distance), time spent for staying in the open arms (Open arm duration) and number of entries into the open arms (Number of open arm entries) in an elevated plus maze test. The values of these parameters were decreased after social defeat stress compared to the values before social defeat stress. No significant difference was found among mice injected with AAV‐hrGFP and mice injected with AAV‐FGF21. B, Total distance of locomotion and time spent for social contact with ICR mice in a social avoidance test. Total locomotor distance and time spent for investigating a cylinder containing an ICR mouse were reduced after social defeat stress, and no significant difference was found among mice injected with AAV‐hrGFP and mice injected with AAV‐FGF21. C, Total distance of locomotion and time spent for social contact with a C57BL/6J mouse in a social avoidance test. Total locomotor distance and time spent for investigating a cylinder containing a C57BL/6J mouse were reduced after social defeat stress, and no significant difference was found among mice injected with AAV‐hrGFP and mice injected with AAV‐FGF21. The number of mice injected with AAV‐hrGFP, 5 × 10⁸ vg AAV‐FGF21 and 1 × 10⁹ vg AAV‐FGF21 was 12, 13 and 12, respectively. The number of wild‐type mice and FGF21‐deficient mice was 6 and 8, respectively. ††††P < 0.0001, before stress vs after stress, two‐way repeated measures ANOVA