FIG. 3.

The influence of the incubation temperature on the cyanophycin synthetase-catalyzed incorporation of arginine. (a) Cyanophycin synthetase activity as a function of the incubation temperature. The enzyme activity was determined for 4 min after start of the reaction at the indicated temperature. (b) Time courses of the incorporation of arginine into cyanophycin at 28°C (▫) and 50°C (●). The incorporated amount of arginine was determined at the specified time points in 125 μl of complete reaction mixture, as described in Materials and Methods. Purified cyanophycin synthetase was used for these experiments.