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Purification of cyanophycin synthetase
| Purification step | Volume (ml) | Protein (mg) | Activity (U)a | Sp act (U · mg of protein−1) | Purification (fold) | Yield (%) |
|---|---|---|---|---|---|---|
| Soluble cell fraction | 54 | 5,151 | 17,095 | 3.32 | 1 | 100 |
| Q Sepharose | 44 | 162.3 | 9,747 | 60 | 18 | 57 |
| Procion Blue | 9 | 17.46 | 2,653 | 152 | 46 | 15.5 |
| Superdex 200 | 6 | 5.28 | 1,257 | 238 | 72 | 7.3 |
Units are nanomoles of arginine incorporated into cyanophycin per minute.
