TABLE 3.

Formation of ADP during cyanophycin synthetase-catalyzed incorporation of amino acid substrate^a

Condition	ADPb (mM)	Argininec incorporated (mM)	Aspartic acidc incorporated (mM)
Complete	0.56	0.216	0.202
Without aspartic acid	0.04	0.003	N.D.d
Without arginine	0.07	N.D.	0.005
Without ATP	<0.02	<0.0005	N.D.
Without cyanophycin	0.05	0.003	N.D.
Without enzyme	<0.02	<0.0005	N.D.
Without MgCl2	<0.02	<0.0005	N.D.

^aData represent the mean of four determinations. 

^bThe ADP content in the reaction mixture and the amount of amino acids incorporated into cyanophycin were determined after 1 h of incubation at 28°C, as described in Materials and Methods. 

^cThe incorporation of arginine and aspartic acid was determined in separate runs. 

^dN.D., not determined.