TABLE 3.
Condition | ADPb (mM) | Argininec incorporated (mM) | Aspartic acidc incorporated (mM) |
---|---|---|---|
Complete | 0.56 | 0.216 | 0.202 |
Without aspartic acid | 0.04 | 0.003 | N.D.d |
Without arginine | 0.07 | N.D. | 0.005 |
Without ATP | <0.02 | <0.0005 | N.D. |
Without cyanophycin | 0.05 | 0.003 | N.D. |
Without enzyme | <0.02 | <0.0005 | N.D. |
Without MgCl2 | <0.02 | <0.0005 | N.D. |
Data represent the mean of four determinations.
The ADP content in the reaction mixture and the amount of amino acids incorporated into cyanophycin were determined after 1 h of incubation at 28°C, as described in Materials and Methods.
The incorporation of arginine and aspartic acid was determined in separate runs.
N.D., not determined.