TABLE 5.
Number of study subjects who tested positive for Helicobacter spp. based on PCR a as well as serological tests
| Presence of Helicobacter spp. DNA based on PCR detection | Positive for serum anti‐H. bilis IgG antibodies | Positive for serum anti‐H. hepaticus IgG antibodies | Positive for serum anti‐H. pylori IgG antibodies | |
|---|---|---|---|---|
| Biliary tract cancer group | ||||
| H. bilis | 2 | 0 | 0 | 2 |
| H. hepaticus | 2 | 1 | 1 | 2 |
| H. pylori, Helicobacter sp., or Campylobacterales | 5 b | 4 | 3 | 4 |
| Pancreatic cancer group | ||||
| H. bilis | 6 | 4 | 2 | 2 |
| H. hepaticus | 1 | 1 | 1 | 0 |
| H. pylori, Helicobacter sp., or Campylobacterales | 12 b | 6 | 5 | 6 |
| Control group | ||||
| H. bilis | 3 | 2 | 1 | 2 |
| H. hepaticus | 1 | 0 | 0 | 1 |
| H. pylori, Helicobacter sp., or Campylobacterales | 3 c | 0 | 0 | 1 |
a
PCR amplification product of 16S rRNA gene was analyzed by direct sequencing. Sequence homology was determined with the basic local alignment search tool (BLAST).
b
Presence of species‐specific cdtB gene was also determined based on PCR. H. bilis‐specific cdtB gene was detected in 2 of 5 biliary tract cancer cases, and 5 of 12 pancreatic cancer cases. Sequence homology was determined with BLAST.
c
H. hepaticus‐specific cdtB gene was detected in 2 of 3 controls. Sequence homology was determined with BLAST.