Figure 3.

Milk‐derived EVs were taken up by chondrocytes and reduced catabolic gene expression in vitro. A) Chondrocytes in monolayer can take up fluorescently labelled (Green, PKH‐67) milk‐derived EVs after 24 h. Nuclei were stained using DAPI (Blue). B) 48‐h stimulation with CMEVs resulted in reduced MMP‐expression. C) ADAMTS‐5 expression. D) TIMP‐3 expression. Gene expression is plotted as fold change of CMEVs over PBS control. Data is shown from one representative experiment out of three individual experiments, performed in technical triplicates. Error bars represent mean ±SD, *p>0.05, **p>0.005, ***p>0.0005.