TABLE 1.

Different enzymatic digestion conditions included in the reviewed protocols. Concentrations are shown as described in the protocols, temperatures are indicated in Celsius, incubation times are presented in minutes. Missing values indicated by (‐).

Protocol source	Enzyme	Concentration	Serial/combinatory	Incubation time	Temperature
Zhang and Hu. 2013 25	Collagenase IV Trypsin	1 mg/ml 0.05%	Serial	15 min 10 min	37°C water bath shaker
Smith et al. 2013 27	Collagenase II Trypsin	1.3 mg/ml 0.05%	Serial	60 min 7 min	37°C water bath shaker
Lowette et al. 2014 28	Collagenase Protease Albumin	14.67 mg/ml 10 mg/ml 5% in PBS	Combinatory	8 min	37°C
Wahba et al. 2016 29	Collagenase IV Trypsin	1 mg/ml 0.05%	Serial	15 min 10–15 min	37°C water bath manual rotation
Brun and Akbarali. 2018 26	Collagenase I DNase I	0.5 mg/ml 0.5 mg/ml	Serial	35 min	37°C water bath shaker
Verissimo et al. 2019 31 EGCs	Collagenase II DNase I	‐	Combinatory	60 min	37°C
Wang et al. 2018 30 EGCs	Non‐enzymatic digestion	‐	‐	‐	‐