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. 2020 Sep 17;130(1):e106. doi: 10.1002/cpim.106

Table 2.

Intestinal Differentiation Media and Expected Cell‐Type Compositions a , b

Medium Reference Expected outcome Special considerations
ENR Sato et al. (2011) Enterocytes, EECs, TACs, goblet cells Generic differentiation medium for broad coverage of differentiated cell types, with particular enrichment of enterocytes
ENR+Notchi Goblet cells Goblet cell differentiation
ENR+MEKi+Notchi Beumer et al. (2018) EECs (crypt/lower villus state) Enteroendocrine cell differentiation inducing a crypt hormone profile
ER+MEKi+Notchi+ BMP4+BMP2 Beumer et al. (2018) EECs (upper villus state) Enteroendocrine cell differentiation inducing an upper villus hormone profile
a

For full composition of media, see Reagents and Solutions.

b

Abbreviations: BMP, bone morphogenetic protein; EEC, enteroendocrine cell; ENR, EGF‐Noggin‐Rspo1; MEK, p38 MAP kinase; TAC, transit‐amplifying cell.