Figure 5.

DRG electrical activity can be modulated by the Ti₃C₂T_x film. (A) Schematic representing a photothermal stimulation event at the interface between DRG neurons and the Ti₃C₂T_x film. (B) Bright field and time series fluorescence images of a representative DRG neuron interfaced with a Ti₃C₂T_x film and labeled with a Ca²⁺ indicator (CalBryte 520 AM). A 635 nm laser pulse of 10 mW power and 1 ms pulse duration was applied at t = 5.6 s. Red arrow indicates the laser target spot. The white circles denote the ROIs for fluorescence intensity analysis. Scale bars are 20 μm. (C) Normalized Ca²⁺ fluorescence intensity as a function of time for the ROIs marked in B. Red arrow denotes the starting point of the applied laser pulse (t = 5.6 s).