FIGURE 3.

Inhibition of PKC and NOTCH mitigates laminin alpha-3 transcriptional changes to key desmosomal and hemidesmosomal genes. Primary human keratinocytes were incubated with laminin alpha-3 blocking antibody (clone: P3H9-2) or isotype control in the following pretreatment with the PKC inhibitor GO6983 and the NOTCH inhibitor DAPT at a concentration of 5μM and 10μM respectively (n=3/condition). RNA-seq was subsequently performed. Expression of select A) desmosomal and B) hemidesmosomal genes is shown for each condition expressed as normalized fragments per kilobase million (FPKM), revealing PKC and NOTCH-dependent inhibition of P3H9-2-mediated keratinocyte differentiation