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. 2020 Mar 5;30(3):e2099. doi: 10.1002/rmv.2099

Table 1.

Genomic characteristics of 22 human clade 2.3.4.4 A(H5N6) viruses

Year of isolation Virus strainsa , b
Sichuan/26221 Changsha/1, Gz/39715, Gd/99710 Yunnan/0127, Yunnan/DQ001, Yunnan/DQ002, Shenzhen/1/15, Shenzhen/TH001, Yunnan/14563, Yunnan/14564, Gd/SZ872, Gd/ZQ874 Shenzhen/TH002, Shenzhen/TH003, Shenzhen/1/16 Anhui/33162, Anhui/33163, Hubei/29578 Hunan/55555, Guangxi/55726 Fujian‐Sanyuan/21099
2014 2015 2016 2017
Gene Phenotype Amino acid position Amino acid substitution
HAc Glycosylation site at 158 N158D N N N N N N N
T160A A A A A S (Anhui/33162, Anhui/33163), A (Hubei/29578) A A
Receptor binding specificityd N186K N N N N N N N
N193K N N N N N D N
Q196R K K K K K K K
N224 N N N N N N N
Q226L Q Q Q Q Q Q Q
G228S G G G G G G G
S227N R R S (Shenzhen1/15, Shenzhen/TH001, Gd/SZ872), R (the others) S S R R
S227R
T318I T T T T T T T
Cleavage site 335‐348 RERRRKR RERRRKR RERRRKR RERRRKR RERRRKR RERRRKR REKRRKR
NAe Oseltamivir resistance H274Y H H H H H H H
N294S N N N N N N N
Stalk deletion No 58–68 deletion
PB2 Increased pathogenicity in mice E627K E E (Changsha/1), K (Gz/39715, Gd/99710) E (Shenzhen/1/15, Shenzhen/TH001, Gd/SZ872, Gd/ZQ874), K (the others) K E (Hubei/29578), K (Anhui/33162, Anhui/33163) E E
D701N N D D D D D D
Q591K Q Q Q Q Q Q Q
T/I271A T T T T T T T
558 V E E E E E E E
PB1 Increased replication in mammalian cells L473V V V V V V V V
L598P L L L L L L L
PB1‐F2 Increased pathogenicity in mice 57 11 11 (Gd/ZQ874), 90 (the others) 90 (Shenzhen/TH002, Shenzhen/1/16), 76 (Shenzhen/TH003) 34 (Hubei/29578), 90 (Anhui/33162, Anhui/33163) 90 90
PA Increased replication in mice A36T A A A A A A A
M1 Increased pathogenicity in mice N30D N N N N N N N
T215A A A A A A A A
M2 Adamantane resistance S31N S S S (Gd/ZQ874), N (the others) N N S S
NS Increased pathogenicity in mice P42S S S S S S S S
Deletion of aa 80‐84 Yes Yes Yes (Gd/ZQ874), No (the others) No No Yes Yes
Increased pathogenicity in mice D92E E E E (Gd/ZQ874), D (the others) D D E D
Presence of PDZ domain Yes Yes Yes (Gd/ZQ874), No (the others) No No Yes Yes
a

Font styles represent genetic groups to which the viruses tested belong; italic underline, A/Sichuan/26221/2014 group; italic, A/Hubei/29578/2016 group; underline, A/Fujian‐Sanyuan/21099/2017 group.

b

Sichuan/26221, A/Sichuan/26221/2014; Gd/99710, A/Guangdong/99710/2014; Gz/39715, A/Guangzhou/39715/2014; Changsha/1, A/Changsha/1/2014; Yunnan/0127, A/Yunnan/0127/2015; Yunnan/DQ001, A/Yunnan/DQ001/2015; Yunnan/DQ002, A/Yunnan/DQ002/2015; Shenzhen/1/15, A/Shenzhen/1/2015; ShenZhen/TH001, A/ShenZhen/TH001/2015; Yunnan/14563, A/Yunnan/14563/2015; Yunnan/1456, A/Yunnan/14564/2015; Gd/SZ872, A/_Guangdong_/SZ872/2015; Gd/ZQ874, A/_Guangdong_/ZQ874/2015; Shenzhen/1/16, A/Shenzhen/1/2016; Shenzhen/TH003, A/Shenzhen/TH003/2016; Shenzhen/TH002, A/Shenzhen/TH002/2016; Hunan/55555, A/Hunan/55555/2016; Guangxi/55726, A/Guangxi/55726/2016; Hubei/29578, A/Hubei/29578/2016; Anhui/33162, A/Anhui/33162/2016; Anhui/33163, A/Anhui/33163/2016; Fujian‐Sanyuan/21099, A/Fujian‐Sanyuan/21099/2017.

c

Amino acid positions of HA protein are designated by H3 numbering.

d

Amino acid substitution Q226L/G228S and N224K/Q226L in HA are responsible to increase the ability to bind to human‐type receptors in combination.

e

Amino acid positions of NA protein are designated by N2 numbering.