Fig. 3.

Socially induced freezing behavior in the SAM is transferred to the non-social OF Test in Stay mice. A) Stay mice treated with intra-BLA infusion of an Orx₂R antagonist, but not an Orx₁R antagonist, experience enhanced freezing in the SAM (Drug Effect, F_5,70 = 9.6, p < 0.001; Phenotype Effect, F_1,70 = 17.6, p < 0.001). Further, mice in Orx_A and Orx₁R stimulation groups exhibit enhanced freezing, while animals treated with an Orx₂R agonist demonstrate significantly reduced freezing in the SAM. B) Antagonism of Orx₁R receptors in the BLA reduced generalized OF Test freezing in Escape mice only, while Orx₂R antagonist treatment increased OF freezing in Stay animals (Drug Effect, F_5,70 = 3.3, p ≤ 0.011; Phenotype Effect, F_1,70 = 21.4, p < 0.001). Additionally, freezing in the OF Test was increased in Orx₁R stimulation group mice, while intra-BLA agonism of Orx₂R reduced freezing in both phenotypes. C) In vehicle-treated control Stay mice, socially induced freezing in the SAM is positively correlated to OF Test freezing (F_1,11 = 8.7, R² = 0.4423, p ≤ 0.0131). ⁻p ≤ 0.05 for comparisons between phenotypes in the same treatment group; ⁺p ≤ 0.006 for comparisons to Vehicle-treated mice of the same phenotype; ^{^}p ≤ 0.006 for comparisons to Orx₁R Ant. group of the same phenotype; ^#p ≤ 0.006 for comparisons to Orx₂R Ant. group of the same phenotype; ^!p ≤ 0.006 for comparisons to Orx_A treatment animals of the same phenotype; ^$p ≤ 0.006 for comparisons to mice in the Orx₁R stimulation group of the same phenotype.