FIG. 1.

TGGE separation of the 16S rDNAs of fast-growing mycobacteria in eight soil samples. Lane 135, Mycobacterium sp. strain 135; lanes AC, CC, JC, and KC, less contaminated soil samples AC, CC, JC, and KC, respectively; lanes AT, CT, JT, and KT, heavily contaminated soil samples AT, CT, JT, and KT, respectively. TGGE bands 1, 3, 4, 9, 24, 10, 11, 12, 13, 14, 15, 17, 18, 19, 22, and 23 correspond to bands AC-1, AT-3, CC-4, CT-9, CT24, CT-10, CT-11, CT-12, JC-13, JC-14, JT-15, KC-17, KC-18, KT-19, KT-22, and KT-23, respectively. Electrophoresis was carried out with a temperature gradient of 55 to 65°C for 16 h 40 min in a 9 M urea–6% gel.