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. 2001 May;67(5):2222–2229. doi: 10.1128/AEM.67.5.2222-2229.2001

FIG. 4.

FIG. 4

Pyrene mineralization curves and TGGE analysis of contaminated soils. (A, C, E, and G) Pyrene mineralization curves. AT, CT, JT, and KT are soils amended with pyrene only. AT+135, CT+135, JT+135, and KT+135 are soils amended with pyrene and Mycobacterium sp. strain 135. Ster AT+135, Ster CT+135, Ster JT+135, and Ster KT+135 are sterilized soils amended with pyrene and strain 135. Soils were sampled for TGGE analysis at days 4, 26, and 80 (asterisks). Error bars represent standard errors from three replicates. (B, D, F, and H) TGGE analysis. Lanes AT, CT, JT, and KT, unamended soil at day 0; lanes, 4, 26, and 80, pyrene-amended soils extracted at days 4, 26, and 80, respectively. Bands CT-25, KT-26, and KT-27 were excised for sequencing and phylogenetic analysis. Electrophoresis was carried out with a temperature gradient of 55 to 65°C for 16 h 40 min in a 9 M urea–6% gel.