Table 1.
Quantitative real‐timepolymerase chain reaction (qRT‐PCR) primer sequences
| Transcript | Genbank acc. # | Position | Forward primer sequence (5′–3′) | Reverse primer sequence (5′–3′) |
|---|---|---|---|---|
| aanat2 | NM_131411.2 | 200–392 | ACGTTACCGGCCAGCGAGTTT | TGATGAAGGCCACCAGCTGCC |
| actb2 | NM_181601.5 | 32–221 | CACACGCAGCTAGTGCGGAA | GCGACCCACGATGGATGGGAA |
| agrp2 | NM_001271291.1 | 176–305 | ACGTGGCGCACCAAGGATCT | TCGGGCCTGGGCTTAGGTCT |
| bsx | NM_214727.1 | 218–412 | TTGCCTCCCGGATGCCTCTTT | TTCTGGCCTTTCTGCGTCTGC |
| crx | NM_152940.1 | 464–553 | CGCCTCAGGAATGGACCTGC | GTGCGAGTGAAGGTGGTGCG |
| eef1a1 | NM_131263.1 | 15–135 | TACCTGGCAAAGGGGAGCAGC | ACTTTCCGGAGTCGACGTGGC |
| exorh | NM_131212.2 | 860–1036 | TCTTCGCTAACCAGGGCGCA | TGCTCTCATCCTCGGCCAGC |
| otx5 | NM_181331.3 | 258–330 | ACCGGGATGGATCTCCTGCACT | TGGTTCTTTCGCGCCGCTGT |
| rho | NM_131084.1 | 613–805 | CTGGTGGGCTGGTCGCGTTA | CGTGCCGCCTCCTTCACAGT |