Figure 15.
Super resolution imaging of a protein with DNA platform‐enabled expansion microscopy. Top: design strategy to anchor Hfq‐like protein onto a rectangular DNA origami substrate for DNA‐PAINT analysis. The protein was chemically conjugated to ssDNA tethers on six ends, which hybridized with short extending DNA strands on the DNA platform. The DNA tethers also contained domains to act as docking strands for imager strand binding in DNA‐PAINT. Bottom: representative results of super‐resolution DNA‐PAINT imaging of the protein‐DNA platform complex. DNA‐PAINT showed colocalized platform (green from ATTO532 dye) and the protein monomers (red from ATTO655 dye). Also shown is a histogram of the interdye distance between ATTO655 per platform to show the intermonomer distance within each protein. Reproduced with permission.27 Copyright 2018, The Royal Society of Chemistry.