Figure 23.

Design and characterization of a dynamic DNA origami “nanorobot” loaded with thrombin (Th) molecules within a rectangular DNA structure and locked into a tubular structure using aptamer locks. Inset shows the side view of a closed nanorobot with aptamer‐DNA duplex (called fastener), which opened in the presence of nucleolin protein into a rectangular sheet. Representative AFM images show the closed and open configurations. Scale bar = 100 nm. Whole animal optical analysis after the administration of Th‐loaded nanorobot to tumor‐bearing mice at t = 0. Tumor volume tracked over 21 d after the administration with saline, free Th, targeted empty nanorobot, nontargeted nanotube‐Th, targeted nanotube‐Th or nanorobot‐Th (nanorobot‐Th vs targeted nanotube‐Th, p = 0.00016; targeted nanotube‐Th vs saline, p = 0.00031). Reproduced with permission.[[qv: 48c]] Copyright 2018, Springer Nature.