TABLE 1.
Measured size data, theoretical MW data, derived MW data and mass spectrometric data of empty and filled AAV8 VLP preparation
| AAV8 VLP preparations | ||
|---|---|---|
| Investigative approach: | Empty | Filled |
| nES GEMMA EMD data (nm) | 25.10 ± 0.18 | 25.93 ± 0.07 |
| Theoretical MW based on 1:1:10 VPs ratio (kDa) a | 3746 | 5076 |
| Theoretical MW based on SDS‐PAGE (kDa) | 3658 | 4988 |
| EMD/MW correlations (kDa) | 3670 ± 69 | 4751 ± 47 |
| Native MS (kDa) (for n = 161 charges) | 3710 | 5005 |
Abbreviations: EMD, electrophoretic mobility diameter; nES GEMMA, native nano‐electrospray gas‐phase electrophoretic mobility molecular analyzer; MS, mass spectrometry.
a
Based on the following MW: VP1 81 kDa; VP2 65 kDa; VP3 60 kDa. 48