Figure 2.

Scc2 mutants have misregulated gene expression profiles

(A) Few genes were differentially expressed after 3 h of auxin treatment while hundreds of genes were differentially expressed with auxin treatment extended to 9 h. RNA sequencing was performed in Scc2^degron strains containing a plasmid copy of WT, null, R787G, or G1242V SCC2.

(B) The gene expression profile of scc2^R787G is similar to null, whereas scc2^G1242V clusters more closely to WT. Heatmap showing the log₂ transformed read counts per million (CPM) of the differentially expressed genes in the null strain after 9 h auxin treatment.

(C) Differentially expressed genes in scc2^R787G, scc2^G1242V, and null compared with WT are enriched for GO terms translation and ribosome biogenesis for upregulated genes and oxidative stress for downregulated genes.

See also Figure S2.