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. 2022 Jul 5;18(7):e1010307. doi: 10.1371/journal.pgen.1010307

Fig 3. Effects of SOX2 knockout on bovine early embryonic development.

Fig 3

A. sgRNAs used to target SOX2. Red lines represent the position of introduced premature stop codon. B. Experimental design to explore the effects of SOX2 KO on bovine early embryonic development. C. Representative genotyping results for three distinct sgRNAs. WT: wild-type; KO: putative SOX2 knockout embryos. Red arrows denote successful C: T conversion. D. Statistical analysis of editing efficiency for each sgRNA. The target sequence of sgRNA1, sgRNA2 and sgRNA3 were analyzed in 93, 113 and 114 embryos, respectively. E. Immunostaining validation for SOX2 KO at D7.5 blastocyst (BL) stages (Three replicates of 3–5 embryos were analyzed per group). Scale bar = 50 μm. F. Blastocyst formation rate of bovine embryos after SOX2 KO. The rate of blastocysts at D7.5 was recorded with no significant difference found between WT and KO groups (Five independent replicates of 20–25 embryos per group). Red asterisks represent hatching blastocysts. Scale bar = 100 μm. G and H: Statistical analysis of total cell numbers at D7.5 (G) and D8.5 (H). Asterisks refer to significant differences (*:P < 0.05; **:P <0.05; ***: P<0.001).