Table 1. Oligonucleotide primer sequences for RT-qPCR.
| Gene | Sequence | Accession number | Reference |
|---|---|---|---|
| ACC | F: CACTTCGAGGCGAAAAAC | XM_015295697.2 | [27] |
| R: GGAGCAAATCCATGACCA | |||
| CAT | F: ACCAAGTACTGCAAGGCGAA | NM_001031215.1 | [27] |
| R: TGAGGGTTCCTCTTCTGGCT | |||
| EXFABP | F: GGAGGACCTTGCACATGA | NM_205422.1 | This study |
| R: GTGTAGTTCCGCTCCCTA | |||
| FAS | F: CAATGGACTTCATGCCTC | NM_205155.3 | [27] |
| R: GCTGGGTACTGGAAGACA | |||
| GPx1 | F: AACCAATTCGGGCACCAG | NM_001277853.2 | [27] |
| R: CCGTTCACCTCGCACTTCTC | |||
| HSP70 | F: CCCGAGCAAGCTGGATTCT | AY143693.1 | [27] |
| R: CAGGAGCAGATCTTGCACATTT | |||
| HSP90 | F: GCGAAGACGTGTTCCTGTAT | NM_001109785 | [22] |
| R: GGTCATCCCTATGCCGGTATC | |||
| NOX1 | F: GCGAAGACGTGTTCCTGTAT | NM_001101830.1 | [22] |
| R: GAACCTGTACCAGATGGACTTC | |||
| NOX4 | F: CCTCTGTGCTTGTACTGTGTAG | NM_001101829.1 | [22] |
| R: GACATTGGAGGGATGGCTTAT | |||
| NRF2 | F: CAGAAGCTTTCCCGTTCATAG-A | NM_205117 | This study |
| R: GACATTGGAGGGATGGCTTAT | |||
| PPAR.G | F: TCAGGTTTGGGCGAATGC | XM_040646063.1 | This study |
| R: CGCTCGCAGATCAGCAGA | |||
| SOD | F: AGGGGGTCATCCACTTCC | NM_205064.1 | [22] |
| R: CCCATTTGTGTTGTCTCCAA | |||
| β-actin | F: ACCGGACTGTTACCAACA | NM_205518.1 | This study |
| R: GACTGCTGCTGACACCTT | |||
| GAPDH | F: TTGGCATTGTGGAGGGTCTTA | NM_204305.1 | [27] |
| R: GTGGACGCTGGGATGATGTT |
Abbreviations: ACC, acetyl-CoA carboxylase; CAT, catalase; EXFABP, extracellular fatty acid-binding protein; FAS, fatty acid synthase; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; GPx1, HSP70, heat-shock protein 70; HSP90, heat shock protein 90; glutathione peroxidase 1; NOX1, nicotinamide adenine dinucleotide phosphate oxidase 1; NOX4, nicotinamide adenine dinucleotide phosphate oxidase 4; NRF2, nuclear factor erythroid 2-related factor 2; PPAR-γ, peroxisome proliferator-activated receptor gamma; SOD, superoxide dismutase.