FIGURE 7.
EP1 receptor antagonism attenuates late stage fibrosis in human precision‐cut kidney slices (PCKS). Human PCKS were incubated with TGF‐β (10 ng/mL) in the absence or presence of SC‐19220 (225 µM) for 24 and 48 hours. (A, B) Gene expression of fibronectin (FN), alpha‐smooth muscle actin (αSMA) and collagen 1a1 (Col‐1a1) was studied by qPCR in fibrotic slices incubated for 24 (A) and 48 hours (B). Relative expression was calculated using the reference gene RPL22 (n = 6). (C) Viability of PCKS after treatment with SC‐19220, assessed by the ATP content of the slices (n = 6). Data are presented as means ± SEM; *P < .05 compared to control. § P < .0507 compared to control. # P < .056 compared to control