TABLE 4.
Primers used for qPCR
| Target gene | Species | Direction | Sequence |
|---|---|---|---|
| EP1 | Mouse |
Forward: Reverse: |
5′‐CGGCATTAGTGTGCAATACG‐3′ 5′‐TGGCTGAAGTGATGGATGAG‐3′ |
| EP2 | Mouse |
Forward: Reverse: |
5′‐ATGCTCCTGCTGCTTATCGT‐3′ 5′‐AGGGCCTCTTAGGCTACTGC‐3′ |
| EP4 | Mouse |
Forward: Reverse: |
5′‐CCATCGCCACATACATGAAG‐3′ 5′‐TGCATAGATGGCGAAGAGTG‐3′ |
| FN | Mouse |
Forward: Reverse: |
5′‐AATGGAAAAGGGGAATGGAC‐3′ 5′‐CTCGGTTGTCCTTCTTGCTC‐3′ |
| 18S | Mouse |
Forward: Reverse: |
5′‐TGTGGTGTTGAGGAAAGCAG‐3′ 5′‐TCCCATCCTTCACATCCTTC‐3′ |
| Col 1a1 | Mouse |
Forward: Reverse: |
5′‐CACCCTCAAGAGCCTGAGTC‐3′ 5′‐ACTCTCCGCTCTTCCAGTCA‐3′ |
| Col 3a1 | Mouse |
Forward: Reverse: |
5′‐GCACAGCAGTCCAACGTAGA‐3′ 5′‐TCTCCAAATGGGATCTCTGG‐3′ |
| αSMA | Mouse |
Forward: Reverse: |
5′‐CTGACAGAGGCACCACTGAA‐3′ 5′‐CATCTCCAGAGTCCAGCACA‐3′ |
| FN | Human |
Forward: Reverse: |
5′‐CAGTGGGAGACCTCGAGAAG‐3′ 5′‐GTCCCTCGGAACATCAGAAA‐3′ |
| Col 1a1 | Human |
Forward: Reverse: |
5′‐CCTGGATGCCATCAAAGTCT‐3′ 5′‐AATCCATCGGTCATGCTCTC‐3′ |
| αSMA | Human |
Forward: Reverse: |
5′‐ACTGGGACGACATGGAAAAG‐3′ 5′‐TACATGGCTGGGACATTGAA‐3′ |
| RPL‐22 | Human |
Forward: Reverse: |
5′‐GGAGCAAGAGCAAGATCACC‐3′ 5′‐TGTTAGCAACTACGCGCAAC‐3′ |
| EP1 | Human |
Forward: Reverse: |
5′‐TTGTCGGTATCATGGTGGTG‐3′ 5′‐ATGTACACCCAAGGGTCCAG‐3′ |
| EP2 | Human |
Forward: Reverse: |
5′‐CCACCTCATTCTCCTGGCTA‐3′ 5′‐TTCCTTTCGGGAAGAGGTTT‐3′ |
| EP4 | Human |
Forward: Reverse: |
5′‐GACCTGTTGGGCACTTTGTT‐3′ 5′‐AGGTAGCGCTCGACACTCAT‐3′ |
| TGF‐β | Dog |
Forward: Reverse: |
5′‐AAGAAAAGTCCGCACAGCAT‐3′ 5′‐GCTGCTCCGCTTTTAACTTG‐3′ |
| GAPDH | Dog |
Forward: Reverse: |
5′‐AACATCATCCCTGCTTCCAC‐3′ 5′‐GGCAGGTCAGATCCACAACT‐3′ |
| EP1 | Dog |
Forward: Reverse: |
5′‐CTGTGAGCCTCTGCTCCTG‐3′ 5′‐GACAGCCAGCACCACTAACA‐3′ |