TABLE 1.

List of PCR conditions and primers used in this study

Target gene	Primer	Sequence (5′‐3′)	Condition	Cycle	Amplicon (bp)	Reference
pbp1A	PBP1‐F PBP1‐R	5′‐GCATGATCGTTACAGACACG‐3′ 5′‐ATCCACGATTTCTTTACGC−3′	Pre‐heating: 96℃, 2 min Denature: 94℃, 1 min Annealing: 60℃, 1 min Extension: 72℃, 1 min	35	905	15
23S rRNA	Hp23S 1835F Hp23S 2327R	5′‐GGTCTCAGCAAAGAGTCCCT‐3′ 5′‐CCCACCAAGCATTGTCCT‐3′	Pre‐heating: 96℃, 2 min Denature: 96℃, 30 s Annealing: 57℃, 30 s Extension: 72℃, 1 min	35	493	16
gyrA	gyrA F1 gyrA R1	5′‐AAAGCCCGTGCATAGGC‐3′ 5′‐TCCCATTAGCCCCATTGA′‐3′	Pre‐heating: 96℃, 2 min Denature: 96℃, 30 s Annealing: 52℃, 30 s Extension: 72℃, 1 min	35	398	17
KHP30 ORF13/KHP40 ORF14	orf13‐F orf13‐R	5′‐GAAACYTTRAARCGWGARATCGCGCAA‐3′ a 5′‐ACRCCRTTRCCTAACGCTTCTTTAGG‐3′ a	Pre‐heating: 96℃, 2 min Denature: 96℃, 30 s Annealing: 60℃, 30 s Extension: 72℃, 30 s	40	482	18
KHP30 ORF14/KHP40 ORF15	orf14‐F orf14‐R	5′‐ATTTAGARATYTTRAGYCAAACGATCTACCCG‐3′ a 5′‐ACGGTTTCATCAATRTARAAYCTYGTTTCTTT‐3′ a	Pre‐heating: 96℃, 2 min Denature: 96℃, 30 s Annealing: 50℃, 30 s Extension: 72℃, 30 s	40	751	18
KHP30 ORF15/KHP40 ORF16	orf15‐F orf15‐R	5′‐GCRGAAGTSGTRAAGGTGAGYTTAGAA‐3′ a 5′‐ACGCTBGGYAGAAARTAHAACACTCT‐3′ a	Pre‐heating: 96℃, 2 min Denature: 96℃, 30 s Annealing: 50℃, 30 s Extension: 72℃, 30 s	40	269	18

^a B = C + G + T, H = A + C + T, R = A + G, S = C + G, W = A + T, Y = C + T.