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Polyacrylamide gel electrophoresis of MnlI RFLPs from nifH PCR products obtained from LCSA-c soil fractions. Lane 1, >250-μm fraction; lane 2, 250- to 50-μm fraction; lane 3, 50- to 20-μm fraction; lane 4, 20- to 2-μm fraction; lane 5, <2-μm fraction; lane 6, 20-bp molecular size marker. Asterisk, 250-bp fragment characteristic of the <2-μm fraction.
