| 1 cycle: | 45 s | 98°C | (denaturation) |
| 16 cycles: | 15 s | 98°C | (denaturation) |
| 30 s | 65°C | (annealing) | |
| 70 s | 72°C | (extension) | |
| 1 cycle | 2 min | 72°C | (final extension). |
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