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. 2022 Jul 15;8(28):eabl7560. doi: 10.1126/sciadv.abl7560

Fig. 7. Simultaneous imaging of bassoon and RIM2 with 3D-MINFLUX shows that they are intimately colocalized.

Fig. 7.

(A) Confocal image of a rod ribbon AZ stained for bassoon (CF660C) and RIM2 (Alexa 647), without spectral unmixing. (B) The corresponding two-color 3D-MINFLUX image, viewed from the side (z-projection) shows RIM2 slightly interior to bassoon. Scale bars in (A) and (B): 200 nm. Additional images of this ribbon are presented in (C) and (D) as y- and x-projections, respectively. In (E) the image is tilted to better reveal the arrangement of RIM2 and bassoon into two rows. The gray square box in each panel (C to E) has an edge with a length of 100 nm.