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. 2022 Jul 15;8(28):eabl7560. doi: 10.1126/sciadv.abl7560

Table 1. Antibody list.

aa, amino acids; Ms, mouse; ihcs, inner hair cells.

Primary antibody Epitope, species
(sequence database)
Catalog no. and source;
references to mouse
ihc and rod ribbons
Rabbit anti–
ubMunc13-2
aa 182–408 B. Cooper, MPI-NAT,
Goettingen; Ms rods (25)
Mouse anti-bassoon aa 756–1001 mouse
(CAA76598.1)
SAP7F407, Abcam; Ms
rods (51)
Rabbit anti-RIM2 aa 909–1076 rat
RIM2-4C (UniProt ID:
Q9JIS1-3)
140 303, Synaptic
Systems; Ms rods (32)
and Ms ihcs (52)
Mouse anti–ctbp2 aa 361–445 ribeye
(UniProt ID: O54855)
612044, BD Biosciences;
Ms rods (15) and ihcs (14)
Rabbit anti-Cav1.4,
α1F subunit
aa 1667–1985 mouse
Cav1.4 (UniProt ID:
Q7TNI3)
365 003, Synaptic
Systems; Ms rods (53)
Guinea pig anti–
ribeye A-domain
aa 95–207 rat ribeye
(UniProt ID:
Q9EQH5-2)
192 104, Synaptic
Systems; Ms rods (53)
and Ms ihcs (54)
Rabbit anti-CAST aa 655–670 rat ERC2
(UniProt id: Q8K3M6)
143 103, Synaptic
Systems; Ms rods (31)
Guinea pig
anti-piccolo
aa 2012–2351 rat
piccolo (UniProt id:
Q9JKS6)
142 104, Synaptic
Systems; Ms rods (55)
and Ms ihcs (56)
Secondary
antibodies
Source
Goat anti-rabbit,
Alexa 488
A11008, Thermo Fisher
Scientific
Donkey anti-mouse,
Alexa 488
A21202, Thermo Fisher
Scientific
Goat anti-Guinea pig,
Alexa 488
A11073, Thermo Fisher
Scientific
Goat anti-mouse,
Alexa 546
A11003, Thermo Fisher
Scientific
Goat anti-rabbit,
Alexa 647
A21244, Thermo Fisher
Scientific
Donkey anti-mouse,
Alexa 647
A31571, Thermo Fisher
Scientific
Anti-mouse, CF660C Dye: Biotium; coupled
in-house (Abberior)