Table 1. Antibody list.
Primary antibody |
Epitope, species (sequence database) |
Catalog no. and source; references to mouse ihc and rod ribbons |
Rabbit anti– ubMunc13-2 |
aa 182–408 | B. Cooper, MPI-NAT, Goettingen; Ms rods (25) |
Mouse anti-bassoon | aa 756–1001 mouse (CAA76598.1) |
SAP7F407, Abcam; Ms rods (51) |
Rabbit anti-RIM2 | aa 909–1076 rat RIM2-4C (UniProt ID: Q9JIS1-3) |
140 303, Synaptic Systems; Ms rods (32) and Ms ihcs (52) |
Mouse anti–ctbp2 | aa 361–445 ribeye (UniProt ID: O54855) |
612044, BD Biosciences; Ms rods (15) and ihcs (14) |
Rabbit anti-Cav1.4, α1F subunit |
aa 1667–1985 mouse Cav1.4 (UniProt ID: Q7TNI3) |
365 003, Synaptic Systems; Ms rods (53) |
Guinea pig anti– ribeye A-domain |
aa 95–207 rat ribeye (UniProt ID: Q9EQH5-2) |
192 104, Synaptic Systems; Ms rods (53) and Ms ihcs (54) |
Rabbit anti-CAST | aa 655–670 rat ERC2 (UniProt id: Q8K3M6) |
143 103, Synaptic Systems; Ms rods (31) |
Guinea pig anti-piccolo |
aa 2012–2351 rat piccolo (UniProt id: Q9JKS6) |
142 104, Synaptic Systems; Ms rods (55) and Ms ihcs (56) |
Secondary antibodies |
Source | |
Goat anti-rabbit, Alexa 488 |
A11008, Thermo Fisher Scientific |
|
Donkey anti-mouse, Alexa 488 |
A21202, Thermo Fisher Scientific |
|
Goat anti-Guinea pig, Alexa 488 |
A11073, Thermo Fisher Scientific |
|
Goat anti-mouse, Alexa 546 |
A11003, Thermo Fisher Scientific |
|
Goat anti-rabbit, Alexa 647 |
A21244, Thermo Fisher Scientific |
|
Donkey anti-mouse, Alexa 647 |
A31571, Thermo Fisher Scientific |
|
Anti-mouse, CF660C | Dye: Biotium; coupled in-house (Abberior) |