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. 2022 May 17;34(6):e13135. doi: 10.1111/jne.13135

FIGURE 6.

FIGURE 6

NKB mRNA in the arcuate nucleus (ARC) of fed to maintain (FM) and feed‐restricted (FR) ovariectomized (OVX) lambs. Confocal images (20×) of NKB cells (blue) from a FM (A) and a FR (B) OVX lamb. Insets: 1 μm optical sections; 63× objective. (C) Mean ± SEM number of NKB perikarya in the ARC was not significantly different (difference of means = 38.28, SE = 24.96, t 9.7 = 1.53, p = 0.079) between FM (n = 6) and FR (n = 8) OVX lambs. (D) Mean ± SEM integrated density of mRNA for NKB within KNDy neurons was not significantly different (difference of means = −1,112,851, SE = 1,008,531, t 11.7 = −1.12, p = 0.8581) between FM and FR OVX lambs. Scale bars = 50 μm. Inset scale bars = 10 μm. Statistical significance was determined using a one‐tailed, unpaired Students t test