FIGURE 8.
Dynorphin (Dyn) mRNA in the arcuate nucleus (ARC) of fed to maintain (FM) and feed‐restricted (FR) ovariectomized (OVX) lambs. Confocal images (20×) of Dyn cells (red) from a FM (A) and a FR (B) OVX lamb. Insets: 1 μm optical sections; 63× objective. (C) Mean ± SEM number of Dyn perikarya in the ARC was not significantly different (difference of means = 13.35, SE = 34.35, t 11.5 = 0.39, p = 0.352) between FM (n = 6) and FR (n = 8) OVX lambs. (D) Mean ± SEM integrated density of mRNA for Dyn within KNDy neurons was not significantly different (difference of means = −85,149, SE = 172,570, t 12 = −0.49, p = 0.685) between FM and FR OVX lambs. Scale bars = 50 μm. Inset scale bars = 10 μm. Statistical significance was determined using a one‐tailed, unpaired Students t test