FIGURE 6.

Effect of Yoda‐1 on [Ca²⁺] _i . A, In flexor digitorum brevis (FDB) myofibres, Yoda1 (10 μM) did not alter the basal [Ca²⁺] _i . The [Ca²⁺] _i variations induced by high K⁺ depolarisation (KCl 60 mM) and caffeine (Caff 40 mM) were used as positive controls of cell responsiveness to stimuli able to increase the [Ca²⁺] _i . B, In myotubes, at the same concentration, the agonist of Piezo1 channels induced [Ca²⁺] _i oscillations in 35% of the observed myotubes (see text for further details). The effect of Yoda1 was prevented by myotube preincubation with Gd³⁺ (300 μM). Each set of Ca²⁺ imaging experiments was carried out on three independent cell culture preparations